Example of accurate quantification of total cell fluorescence. (a) Quantitative western blot
data for Ste5 (Benjamin, et.al. submitted) for wild type and for strains with the wild type
replaced with 1X, 2X or 4X copies of YFP-Ste5. (b) Histograms of measured fluorescence
for each of these strains as well as a 5X YFP-Ste5 strain. (c) The means of (b) plotted
as a function of YFP-Ste5 copy number. The linearit of the response in (c) indicates that
we are able to accurately quantify small numbers of fluorescent proteins (Benjamin, et.al.,
submitted). Image from Gordon, et.al. 2007.